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本研究旨在获得抗番茄茎腐病的生防菌株，并对其培养基进行优化。采用平板对峙法从番茄根际土样中筛选获得1株抑制番茄茎腐病菌的枯草芽孢杆菌(Bacillus subtilis)7-1,结果显示菌株7-1对番茄茎腐病病原菌的抑菌率可达47%。通过响应面法优化发酵培养基组成后，菌株7-1菌体生物量显著提升，得到最佳培养基配方：木薯淀粉12.0 g/L,豆饼粉5.2 g/L,氯化钠6.1 g/L。该研究可为土壤微生态功能修复与农业绿色生产提供技术支撑。
Abstract：This study aimed to obtain biocontrol strains against tomato stem rot and optimize its culture medium. A Bacillus subtilis strain, designated 7-1, was screened from agricultural soil using a plate confrontation method and exhibited 48% inhibition of the pathogen of tomato stem rot. The fermentation medium composition was subsequently optimized via response surface methodology, resulting in a significant increase in bacterial biomass. The optimal medium formula was obtained as follows: cassava starch 12.0 g/L, soybean cake powder 5.2 g/L, and sodium chloride 6.1 g/L. This research can provide technical support for the restoration of soil micro-ecological functions and green agricultural production.
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基本信息:
DOI：10.16191/j.cnki.hbkx.2025.06.010
中图分类号:TQ920.6;TQ450.1
引用信息:
[1]秦梦,秦艳梅,张飞燕,等.抗番茄茎腐病生防菌株筛选及发酵培养基优化[J].河北省科学院学报,2025,42(06):48-57.DOI:10.16191/j.cnki.hbkx.2025.06.010.
基金信息:
河北省科学院科技计划项目(25309)
2025-12-15
2025-12-15
